89 North OptoSplit III LS Image Splitter

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Easy-to-align image splitter that enables a single camera to record images simultaneously at three different emission wavelengths

  • User-friendly design requires minimal maintenance and makes alignment simple
  • Excellent for use in FRET, TIRF, and spinning disk confocal applications
  • Compatible with all major brands of camera, including large sCMOS chips

The OptoSplit III LS has been designed as a convenient, inexpensive solution for simultaneous multi-wavelength imaging. Traditionally, multi-wavelength imaging experiments required that multiple images be captured sequentially. However, because the various images were not taken at the same time, temporal artifacts, caused by intracellular movement, often appeared between exposures. To eliminate these temporal artifacts and provide the most accurate measurements, the OptoSplit III allows three images to be acquired simultaneously.

The images captured by the OptoSplit III contain information from three different emission bands, and can be processed using commercially available software packages. Alternatively, the images can be processed manually. While it is optimized for coupling to a scientific microscope, the OptoSplit III can also be used with camera lenses or any other system of lenses which produce an image plane of suitable size.


  • Ratiometric, calcium, voltage & pH imaging
  • Triple probe widefield imaging
  • Förster Resonance Energy Transfer (FRET)
  • Total Internal Reflection Fluorescence (TIRF)
  • Spinning disk confocal microscopy
  • Polarisation/Anisotropy
  • Multifocal Plane Microscopy (MUM)
  • Combined fluorescence/transmitted light microscopy


  • Standard Spectral Range: 450 nm – 900 nm
  • Dimensions: 122 mm (L) x 374 mm (W) x 79 mm (H)
  • Weight: 1.80 kg
  • Emission filter size: 25 mm
  • Dichroic mirror size: 
    • 25.5 mm x 36.0 mm OR 26.0 mm x 38.0 mm
    • 2.0 mm thick, UltraFlat dichroics highly recommended

Additional information

Equipment Condition



OptoSplit III LS