Motorized TIRF, oblique and widefield illuminator for single-mode and multi-mode lasers

• Easy to setup, align and run
• Works with single-mode and multi-mode lasers
• Motorized movement of illumination spot
• Joystick or software control
• Motorized widefield bypass mode

The OptoTIRF is a compact and powerful, yet inexpensive, motorized TIRF illuminator designed to fit onto most research-grade inverted microscope. It gives the researcher intuitive and dynamic access to the entire back aperture of the objective with joystick or software control and simple storage and recall of preset positions. This makes it suitable for acquisition protocols involving TIRF and / or oblique illumination at a range of penetrations depths and wavelengths. Full, 360 degree, positioning and the ability to image at multiple points makes it straightforward to tweak the illumination to the sample and minimize fringes or shading gradients. The OptoTIRF also supports multi-mode fibers allowing the use of higher power and less expensive multi-mode lasers for high speed localization microscopy. Flexibility is further enhanced by a motorized tube lens for automatic correction for different height sample dishes, or different magnification TIRF lenses, and a motorized bypass for widefield illumination using LEDs or a liquid light guide.