Andor Zyla 4.2 Plus USB3 sCMOS Monochrome Microscope Camera

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Specifications:

  • Model: Zyla 4.2 PLUS
  • Sensor type: Front Illuminated Scientific CMOS Front Illuminated Scientific CMOS
  • Active pixels (W x H): 2048 x 2048 (4.2 Megapixel)
  • Sensor size: 13.3 x 13.3 mm18.8 mm diagonal
  • Pixel readout rate (MHz):
    • Slow Read 216 (108 MHz x 2 sensor halves)
    • Fast Read 540 (270 MHz x 2 sensor halves)
  • Read noise (e-) Median [rms]: Rolling Shutter
    • @ 216 MHz: 0.90 [1.1]
    • @ 540 MHz: 1.10 [1.3]
  • Maximum Quantum Efficiency: 82%
  • Sensor Operating Temperature:
    • Air cooled: 0ºC (up to 27ºC ambient)
    • Water cooled: -10ºC
  • Dark current, e-/pixel/sec @ min temp:
    • Air cooled: .10
    • Water cooled: .019
  • Readout modes: Rolling Shutter and Global Clear
  • Maximum dynamic range: 33,000:1
  • Photon Response Non-Uniformity (PRNU):
    • Half-light range: < 0.01%
    • Low light range: <0.1%
  • Pre-defined Region of Interest (ROI): 1920 x 1080, 1392 x 1040, 512 x 512, 128 x 128
  • User defined ROI (Granularity):Yes (1 pixel)
  • Data range: 12-bit (fastest USB 3.0 speeds) and 16-bit (maximum dynamic range)
  • Interface: USB 3.0

General Specifications:

  • Pixel size (W x H): 6.5 μm
  • Pixel well depth (e-): 30,000
  • Linearity (%, maximum):
    • Full light range: Better than 99.8%
    • Low light range (< 1000 electrons signal): Better than 99.9%
  • MTF (Nyquist @ 555 nm): 45%
  • Pixel binning: Hardware binning: 2 x 2, 3 x 3, 4 x 4, 8 x 8
  • Anti-blooming factor: x 10,000
  • I/O: External Trigger, Fire, Fire n, Fire All, Fire Any, Arm
  • Trigger Modes: Internal, External, External Start, External Exposure, Software Trigger
  • Software Exposure Events: Start exposure – End exposure (row 1), Start exposure – End exposure (row n)
  • Hardware timestamp accuracy: 25 ns
  • Internal memory: 1 GB

Zyla 4.2 PLUS is the latest sCMOS technology advancement from Andor. At its heart, Zyla 4.2 PLUS benefits from latest generation sCMOS sensor technology, delivering a further 10 % boost in QE, resulting in an unprecedented 82% QEmax.

Zyla 4.2 PLUS benefits from latest generation microlens front-illuminated sCMOS sensor technology from Fairchild Imaging, delivering an impressive 82% QEmax, coupled with sub-electron read noise. The broad QE profile provides excellent coverage of the visible/NIR wavelength range, highly optimized to a wide range of common fluorophores, the sensitivity boost permitting use of shorter exposure times, faster frame rates and reduced phototoxicity.

Superior data transfer efficiency and Zyla’s unique 12-bit high speed mode combine to deliver an incredible 53 fps through the super-convenient USB 3.0 interface, with industry fastest Region of Interest (ROI) frame rates. With this capability, speed is on tap to allow users to follow faster dynamic processes with improved temporal resolution. Opt for the Camera Link version to access up to a blistering 100 fps (full resolution). Zyla 4.2 PLUS also offers industry-leading linearity (99.8%) for unparalleled quantitative accuracy of measurement across the full dynamic range.

The Zyla 4.2 is ideal for applications that benefit from optimal sensitivity and speed, such as calcium imaging, light sheet microscopy and super-resolution microscopy, as well as numerous high speed applications within astronomy. New application specific capabilities include LightScan PLUS, featuring FlexiScan & CycleMax functionality, permitting simple and flexible adaption of the Rolling Shutter scan mode to applications such as Scanning LightSheet Microscopy and Line Scan Confocal. Zyla 4.2 PLUS can also output data at a sustained 26,041 fps from a 2048(h) x 8(v) ROI, ideal for measuring diffusion coefficients in Fluorescence Correlation Spectroscopy (FCS).

The ‘4T’ (4-transistor) design of the sensor pixel permits more photons to enter, driving the front-illuminated Quantum Efficiency up to 82%. The 4T design also means that it is fundamentally a Rolling Shutter sensor. However, a feature called ‘Global Clear’ has been implemented, which allows for a simulated Global Exposure mode, which requires TTL communication between the camera and a pulsed light source, emulating the Global shutter exposure condition.

The Andor GPU Express library has been created to simplify and optimize data transfers from camera to a CUDA-enabled NVidia Graphical Processing Unit (GPU) card to facilitate accelerated GPU processing as part of the acquisition pipeline.

Features:

  • 82% QE: Highest and broadest available sCMOS QE. Optimized to a wide range of common fluorophores. Enables shorter exposures, faster frame rates and reduced photobleaching.
  • 0.9 e- read noise: Lowest read noise sCMOS. Significantly lower than any CCD.
  • Industry Fastest Frame Rates – Follow faster processes. Industry-fastest USB 3.0 frame rates, 53 fps (full frame). 100 fps (full frame) sustained via Camera Link.
  • 4.2 megapixel sensor format and 6.5 µm pixels – Delivers extremely sharp resolution over a 18.8 mm diagonal field of view; ideal for cell microscopy and astronomy.
  • Ultra Low Fan Vibration – Designed with vibration sensitive experiments in mind, such as super-resolution microscopy.
  • LightScan PLUS – Reduce background and improve contrast and resolution in scattering samples. Designed to allow users to optimise signal strength and confocality concurrently in applications such as Scanned Light Sheet Microscopy and Line Scanning Confocal Microscopy.
  • GPU Express – Simplify and optimize data transfers from camera to Graphical Processing Unit (GPU) card to facilitate accelerated GPU processing as part of the acquisition pipeline.
  • Dual-Gain Amplifiers – Maximum well depth and lowest noise simultaneously, affording extended dynamic range of 33,000:1
  • 12-bit and 16-bit modes – 12-bit for absolute fastest frame rates through USB 3.0; 16-bit for full dynamic range.
  • Compact and Light – Ideal for integration into space restrictive set-ups. Ideal for OEM.
  • Water Cooled Option – Zyla 5.5 and Zyla 4.2 available as water cooled variant, for absolute lowest vibration and -10 °C cooling / reduced darkcurrent.
  • TE cooling to 0 ° C in 27 °C ambient – Ideal for OEM integration into enclosed systems.
  • Extensive FPGA on-head data processing – Essential to ensure best image quality and quantitative fidelity from sCMOS technology.
  • Hardware Timestamp – FPGA generated timestamp with 25ns accuracy.
  • Dynamic Baseline – Essential to ensure quantitative accuracy across the image area and between
  • Clamp – Successive images of a kinetic series.

Additional information

Equipment Condition

Brand

Frame Rate

53 fps

Camera Resolution

4.2 Megapixels

Model

Zyla 4.2P USB3

Sensor Type

sCMOS

Color or Monochrome

Monochrome

QE

82%