Single-Sided Systems (iSPIM)
Light sheet created from one objective and imaged using the other objective. The light sheet is moved through the sample, most often by moving the light sheet using the scanner (galvo) which is synchronized with a piezo stage moving the imaging objective.
- Advantages: Fastest acquisition, least expensive, straight-forward set-up.
- Disadvantages: Better XY resolution than Z resolution.
Double-Sided Systems (diSPIM)
Both sides have a light sheet scanner, piezo objective positioner, and camera. During an experiment a stack of images is collected from both views, and the two datasets can be merged computationally to yield a 3D dataset with isotropic resolution (the usual problem of poor axial resolution is overcome by information from the other view). Can operate in single-sided mode if desired.
- Advantages: XY & Z resolutions are all very good – yielding a combination of speed and resolution that is unsurpassed for live cell imaging.
- Disadvantages: More hardware to buy. Data post-processing required for isotropic resolution.