Biorad Chemidoc XRS+ - 1-min
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Biorad Chemidoc XRS Plus Imaging System

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Includes Computer loaded with Image Lab Software 

Specifications:

  • Maximum sample size: up to 28 x 36 cm
  • Maximum image area: up to 26 x 35 cm
  • Maximum image area: (For standard UV-excited gels) 25 x 26 cm
  • Excitation source: Epi-white light and trans-UV (302 nm) are standard (optional 365 nm lamp available); optional trans-white conversion screen and XcitaBlue™ UV/blue conversion screen available
  • Illumination control: 5 modes (trans-UV, epi-white, and no illumination for chemiluminescence are standard); trans white and XcitaBlue conversion screens are optional
  • Detector: Supercooled CCD
  • Pixel size (H x V): 6.45 x 6.45 µm
  • Cooling system: Peltier
  • Camera cooling temperature: -30°C controlled
  • Filter selector: 3 positions (2 for filters, 1 without filter, for chemiluminescence)
  • Emission filters: 1 included (standard), 3 optional
  • Dynamic range: >4.0 orders of magnitude
  • Pixel density (gray levels): 65,535
  • Dynamic flat fielding: Application specific, for all applications
  • Instrument size (L x W x H): 36 x 60 x 96 cm
  • Instrument weight: 32 kg

Operating Ranges:

  • Operating voltage: 110/115/230 V AC nominal
  • Operating temperature: 10–28°C (21°C recommended)
  • Operating humidity: <70% noncondensing

Automation Capabilities: 

  • Workflow automated selection: Application driven, user selected or recalled by a protocol
  • Workflow automated execution: Controlled by a protocol via application-specific setup for image area, illumination source, filter, analysis, and reporting
  • Workflow reproducibility: 100% repeatability via recallable protocols; from image capture to quantitative analysis and reports
  • Autofocus (patent pending): Precalibrated focus for any zoom setting or sample height
  • Image flat fielding: Dynamic; precalibrated and optimized per application
  • Autoexposure: 2 user-defined modes (intense or faint bands)

The ChemiDoc XRS+ System is based on CCD high-resolution, high-sensitivity detection technology and modular options to accommodate a wide range of samples and support multiple detection methods including fluorescence, colorimetry, densitometry, chemiluminescence, and chemifluorescence. The system is controlled by Image Lab software to optimize imager performance for fast, integrated, and automated image capture and analysis of various samples.

The system accommodates a wide array of samples, from large handcast polyacrylamide gels to small ReadyAgarose Gels and various blots. The system is an ideal accompaniment to PCR, purification, and electrophoresis systems, enabling image analysis and documentation of restriction digests, amplified nucleic acids, genetic fingerprinting, RFLPs, and protein purification and characterization.

Key benefits include:

Gel and blot imaging and analysis are quick and accurate
Automated, hands-off routines; no training is required
Save and recall all the steps in the workflow for repeatable and reproducible results
Optimize the system at setup for image data that is always accurate, reproducible, and free of imaging artifacts
Wide range of applications with special accessories to preserve sample integrity for downstream research while ensuring user safety
Comprehensive, automated quantitative analysis of protein and DNA samples in seconds
Customize and organize data in reports
Obtain publication-quality results quickly
The ChemiDoc XRS+ System optimizes reproducibility and reliability of experimental data, enabling quantitative comparisons between different experiments. Using proprietary algorithms, this imaging system is calibrated at setup to ensure:

Images are in focus at all times, regardless of zoom level or sample position
Appropriate flat fielding correction is automatically and consistently applied to image data for every application
Imaging artifacts are automatically corrected
The ChemiDoc XRS+ System offers fast and sensitive chemiluminescence detection in addition to gel and blot documentation of fluorescent and colorimetric samples. The system includes a supersensitive 16-bit CCD camera that is supercooled for detection of faint samples. The ChemiDoc XRS+ system can be used for imaging with a wide variety of applications that require high resolution and sensitivity (such as chemiluminescent western blots).

The ChemiDoc XRS+ System eliminates the need to use costly and unreliable X-ray film technologies while providing quantitative and reproducable data in seconds. The system features a signal accumulation mode (SAM), which guides a user through determining optimum exposure time and capturing a desired image of a chemiluminescent sample.

Applications:

  • Chemiluminescence: Yes
  • Fluorescence: Yes
  • Colorimetry/densitometry: Yes
  • Gel documentation: Yes

Additional information

Equipment Condition

Model

Chemidoc XRS Plus

Brand

Chemiluminescence

Yes

Colorimetry/densitometry

Yes

Fluorescence

Yes

Gel Documentation

Yes