Hamamatsu C9100-13 Image EM EM-CCD Monochrome Camera

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Specifications:

  • Camera head type: Hermetic vacuum-sealed air/water-cooled head
  • Window: Anti-reflection (AR) coatings on both sides, single window
  • AR mask: Yes
  • Imaging device: Electron Multiplier Back-thinned Frame transfer CCD
  • Effective number of pixels: 512 (H) × 512 (V)
  • Cell size: 16 μm (H) × 16 μm (V)
  • Effective area: 8.192 mm (H) × 8.192 mm (V)
  • Pixel clock rate
    • EM-CCD: 11 MHz, 2.75 MHz, 0.69 MHz
    • NORMAL-CCD: 2.75 MHz, 0.69 MHz
  • EM gain (typ.): 1x, 4x to 1200x
  • Ultra low light detection: Photon imaging mode (1, 2, 3)
  • Fastest readout speed:
    • 31.9 frame/s to 404.4 frame/s
    • 417 frame/s (Binning option)
  • Readout noise (r.m.s.) (typ.):
    • EM-CCD:
      • EM Gain 4x:
        • 25 electrons (at 11 MHz)
        • 20 electrons (at 2.75 MHz)
        • 8 electrons (at 0.69 MHz)
      • EM Gain 1200x:
        • 1 electrons max. (at 11 MHz)
        • 1 electrons max. (at 2.75 MHz)
        • 1 electrons max. (at 0.69 MHz)
    • Normal-CCD: 
      • 17 electrons (at 2.75 MHz)
      • 8 electrons (at 0.69 MHz)
  • Full well capacity (typ.): *3 370 000 electrons (max. 800 000 electrons)
  • Analog gain: 1/2 times to 5 times
  • Gain conversion factor (typ.):
    • EM-CCD readout mode: 5.8 electrons/count
    • NORMAL-CCD readout mode: 1.4 electrons/count
  • Cooling method / temperature:
    • Forced air-cooled: 65 °C stabilized (0 °C to + 30 °C)
    • Water-cooled:
      • -80 °C stabilized (Water temperature: + 20 °C)
      • -90 °C stabilized (Water temperature lower than +10 °C)
  • Temperature stability:
    • Forced air-cooled (-65 °C): ±0.03 °C (typ.) (Room temperature: stable at +20 °C)
    • Water-cooled (-80 °C): ±0.01 °C (typ.) (Water temperature: stable at +20 °C) [Operated with circulating water cooler]
  • Dark current (typ.):
    • Forced air-cooled (-65 °C): 0.01 electron/pixel/s
    • Water-cooled (-80 °C):  0.001 electron/pixel/s
  • Exposure time:
    • Internal sync mode: 30.5 ms or more
    • External trigger mode: 10 μs or more
  • A/D converter: 16 bit
  • Output signal / External control: CameraLink
  • Sub-array: Every 16 lines (horizontal, vertical) size, position can be set (refer to the table on the right)
  • Binning: 2×2, 4×4, (8×8, 16×16)
  • External synchronization: Edge trigger, Level trigger, Start trigger, Synchronous readout trigger
  • Trigger output: Available (Delay and pulse length are variable)
  • Image processing features(Real-time): Background subtraction, Shading correction, Recursive filter, Frame averaging, Spot noise reducer
  • EM gain protection: EM warning mode, EM protection mode
  • EM gain readjustment: Available
  • Lens mount: C-mount

The ImagEM camera evolved from the popular C9100-12 electron multiplier CCD camera. This new generation camera incorporates the latest Hamamatsu engineering and technology to provide a high speed readout rate of 32 frames per second at full spatial resolution and 16 bit digitization. With QE over 90 % and cooling down to -90 ̊C, both low noise and high gain are realized in one camera. ImagEM includes dual readout modes to take advantage of these features. In the EM-CCD readout mode, the on-chip gain provides high speed imaging at very low light levels. In the normal CCD readout mode, the low noise readout and deep cooling provide exceptional images even in long integration situations. High dynamic range, high resolution, high signal to noise ratios and high speed are all hallmarks of this new generation EM-CCD camera. In addition, Photon Imaging mode enables ultra low light detection. Other new features included in the ImagEM are real-time image processing (Background subtraction, Shading correction, and a Recursive filter) plus special internal and external synchronization features. It is now possible to optimize frame synchronization for spinning disk confocal microscopes at up to 32 frames per second with the synchronous readout trigger

Features:

  • High quantum efficiency of 90 % at the peak wavelength
  • -90 ̊C cooling with hermetic sealed head(requires water cooling at +10 ̊C)
  • Dual readout mode(EM-CCD readout / NORMAL CCD readout)
  • High EM gain (1200 times)Real time (32 frame/s) readout at full resolution(512 × 512)
  • Ultra low light detection in Photon Imaging mode
  • Flexible external synchronization modes
  • Synchronous readout trigger mode
  • Both fan and water cooling are included (selectable)
  • Image reversal function in the EM-CCD readout
  • Anti-reflection coating on both sides of input window

Applications:

  • Real time imaging of low light fluorescence
  • Intracellular ion measurement
  • Single molecule fluorescence imaging with TIRF microscopy
  • Real time confocal microscopy
  • Luciferase reporter gene assay
  • Luminescence imaging

Additional information

Brand

Color or Monochrome

Monochrome

Cooled or Uncooled

Cooled

Equipment Condition

Frame Rate

32 fps

Fluorescence

Yes

Model

Image EM

Sensor Type

EM-CCD

QE

90%