Confocal laser scanning microscopes are widely used in life sciences for drug development, exploring biological phenomena and many other purposes. The microscopes that are deployed in cutting-edge research laboratories must operate at peak levels of performance as well as be easy to use – requirements that are fully satisfied by the IX83 inverted research microscope and the FLUOVIEW FV3000 confocal laser scanning microscope. We are pleased to present the story behind the development of these state-of-the-art research tools, including the objectives and processes used to achieve their remarkable designs.
The FLUOVIEW FV3000 series of confocal laser scanning microscopes meets some of the most difficult challenges in modern science. Featuring the high sensitivity and speed required for live cell imaging as well as deep tissue observation, the FV3000 confocal microscope enables a wide range of imaging modalities, including macro-to-micro imaging, super resolution microscopy, and quantitative data analysis.
TruSpectral High-Sensitivity Multichannel Imaging
Using proprietary spectral detection technology, the FV3000 confocal microscope’s TruSpectral detectors combine high sensitivity with spectral flexibility to detect even the dimmest fluorophores.
- Up to 3X more light transmission vs. traditional spectral detection technology
- Independently adjustable channels to optimize signal detection for each individual fluorophore
- Lambda scanning mode enables accurate spectral unmixing of complex overlapping fluorescent signals
- Variable barrier filter mode provides simultaneous four-channel image acquisition, with up to sixteen channels in virtual channel mode
Macro-to-Micro Imaging and Super Resolution Microscopy
The FV3000 microscope’s macro-to-micro workflow provides a roadmap for data acquisition, enabling you to see data in context and easily locate regions of interest for higher resolution imaging.
Use a low-magnification 1.25X or 2X objective to quickly capture a large field of view (FOV) map of whole specimens
Identify regions of interest on the Overlay Map, then switch to a higher magnification objective for high-resolution confocal imaging down to 120 nm with Olympus Super Resolution technology (FV-OSR)
Finalize your acquisition and get publication-ready microscope images with TruSight image processing
Hybrid Scanning for High-Speed Imaging and Increased Productivity
The FV3000 Hybrid Scanner provides two scanners in one for enhanced confocal imaging capabilities.
- The FV3000RS hybrid scan unit uses a galvanometer scanner for precision scanning as well as a resonant scanner, ideal for high-speed imaging of live physiological events
- Capture video-rate images with a large FOV using the resonant scanner, featuring speeds from 30 frames per second (fps) at FN 18 all the way up to 438 fps using clip scanning
- Use the resonant scanner to observe fast phenomena, such as a beating heart, blood flow, or calcium ion (Ca2+) dynamics inside cells
- Switch between the galvanometer scanner and resonant scanner with the click of a button